3,058 research outputs found
The molecular cloning and characterisation of cDNA coding for the alpha subunit of the acetylcholine receptor
The published version of this article is available at Oxford Journals in Nucleic Acids Research at
http://nar.oxfordjournals.org/content/10/19/5809.full.pdf+htmlA rare cDNA coding for most of the α subunit of the Torpedo nicotinic acetylcholine receptor has been cloned into bacteria. The use of a mismatched oligonucleotide primer of reverse transcriptase facilitated the design of an efficient, specific probe for recombinant bacteria. DNA sequence analysis has enabled the elucidation of a large part of the polypeptide primary sequence which is discussed in relation to its acetylcholine binding activity and the location of receptor within the plasma membrane.
When used as a radioactive probe, the cloned cDNA binds specifically to a single Torpedo mRNA species of about 2350 nucleotides in length but fails to show significant cross-hybridisation with a subunit mRNA extracted from cat muscle
Optimization viewpoint on Kalman smoothing, with applications to robust and sparse estimation
In this paper, we present the optimization formulation of the Kalman
filtering and smoothing problems, and use this perspective to develop a variety
of extensions and applications. We first formulate classic Kalman smoothing as
a least squares problem, highlight special structure, and show that the classic
filtering and smoothing algorithms are equivalent to a particular algorithm for
solving this problem. Once this equivalence is established, we present
extensions of Kalman smoothing to systems with nonlinear process and
measurement models, systems with linear and nonlinear inequality constraints,
systems with outliers in the measurements or sudden changes in the state, and
systems where the sparsity of the state sequence must be accounted for. All
extensions preserve the computational efficiency of the classic algorithms, and
most of the extensions are illustrated with numerical examples, which are part
of an open source Kalman smoothing Matlab/Octave package.Comment: 46 pages, 11 figure
SCENE TEXT SEGMENTATION BY APPLYING TRIMMED MEDIAN FILTER USING ENERGETIC EDGE
This proposed method is an accurate and strong method for detecting texts in natural scene images. There are many cases that text regions are not clearly noTable.by background regions due to brightness or illumination variations. The proposed scene text finding process finds out the scene text contents from the natural scene images using the sophisticated edge revealing methods, the local directional number feature and linked map generation process. This proposed method takes natural scene image as input and detects the scene text regions. The detected scene text regions are marked for visual identification for human eyes
The impact of a social norms approach onreducing levels of misperceptions aroundsmokefree hospital entrances amongstpatients, staff, and visitors of a NHShospital: a repeated cross-sectional surveystudy
Background: Smoking is a cause of avoidable morbidity and mortality. In the United Kingdom (UK) the national smoking ban inside hospital buildings is widely adhered to. There is a perception it has led to smokers congregating around hospital entrances (Selbie D. 2016, It’s time for a truly smokefree NHS. Public Health Matters Blog. Public Health England). Efforts to shift social norms and create positive smokefree environments might be strengthened by delivering social norms messages. This study explored the impact of a social norms approach campaign to reduce levels of misperceptions surrounding support for smokefree hospital entrances.
Method: Repeated cross sectional study design. Staff, patients, and hospital visitors at Pinderfields National Health Service (NHS) Hospital (Wakefield, United Kingdom (UK)) completed a survey before and after implementation of a public health social norms campaign (n = 481 surveyed before; n = 459 surveyed after). The main outcome measure was difference between perceived and reported levels of support for smokefree hospital entrances.
Results: There were high levels of support for smokefree hospital entrances. The majority of participants agreed that patients (n = 849, 90% agreed), staff (n = 863, 92% agreed), and visitors (n = 850, 90% agreed) should not smoke in the hospital entrance.
Participants underestimated the proportion of others who self-reported keeping the entrance smokefree. Over 90% of respondents reported not smoking in the hospital entrance, but the perception was that between 50 to 75% of hospital staff, patients, and visitors did not smoke in the hospital entrance.
The mean percentage of hospital staff, patients, and visitors who respondents thought did not smoke in entrances was higher for respondents responding after, compared to those responding before, the campaign. There was an overall significant effect of time on attitudes towards smoking in the entrances; in all instances the mean percentage of hospital staff, patients, and visitors the participants believed agreed that hospital entrances should be smokefree was higher for those responding after, compared with before, the campaign.
Conclusions: People hold misperceptions of the proportion of people who choose to smoke in the hospital entrance. The social norms approach campaign was associated with a strengthening of positive social norms. Such campaigns should be considered by Trusts as one evidence-based based tactic to denormalise smoking, increase support for smokefree policies
Gamma-Ray Background from Structure Formation in the Intergalactic Medium
The universe is filled with a diffuse and isotropic extragalactic background
of gamma-ray radiation, containing roughly equal energy flux per decade in
photon energy between 3 MeV-100 GeV. The origin of this background is one of
the unsolved puzzles in cosmology. Less than a quarter of the gamma-ray flux
can be attributed to unresolved discrete sources, but the remainder appears to
constitute a truly diffuse background whose origin has hitherto been
mysterious. Here we show that the shock waves induced by gravity during the
formation of large-scale structure in the intergalactic medium, produce a
population of highly-relativistic electrons with a maximum Lorentz factor above
10^7. These electrons scatter a small fraction of the microwave background
photons in the present-day universe up to gamma-ray energies, thereby providing
the gamma-ray background. The predicted diffuse flux agrees with the observed
background over more than four decades in photon energy, and implies a mean
cosmological density of baryons which is consistent with Big-Bang
nucleosynthesis.Comment: 7 pages, 1 figure. Accepted for publication in Nature. (Press embargo
until published.
Lepton Acceleration in Pulsar Wind Nebulae
Pulsar Wind Nebulae (PWNe) act as calorimeters for the relativistic pair
winds emanating from within the pulsar light cylinder. Their radiative
dissipation in various wavebands is significantly different from that of their
pulsar central engines: the broadband spectra of PWNe possess characteristics
distinct from those of pulsars, thereby demanding a site of lepton acceleration
remote from the pulsar magnetosphere. A principal candidate for this locale is
the pulsar wind termination shock, a putatively highly-oblique,
ultra-relativistic MHD discontinuity. This paper summarizes key characteristics
of relativistic shock acceleration germane to PWNe, using predominantly Monte
Carlo simulation techniques that compare well with semi-analytic solutions of
the diffusion-convection equation. The array of potential spectral indices for
the pair distribution function is explored, defining how these depend
critically on the parameters of the turbulent plasma in the shock environs.
Injection efficiencies into the acceleration process are also addressed.
Informative constraints on the frequency of particle scattering and the level
of field turbulence are identified using the multiwavelength observations of
selected PWNe. These suggest that the termination shock can be comfortably
invoked as a principal injector of energetic leptons into PWNe without
resorting to unrealistic properties for the shock layer turbulence or MHD
structure.Comment: 19 pages, 5 figures, invited review to appear in Proc. of the
inaugural ICREA Workshop on "The High-Energy Emission from Pulsars and their
Systems" (2010), eds. N. Rea and D. Torres, (Springer Astrophysics and Space
Science series
Conceptualizing pathways linking women's empowerment and prematurity in developing countries.
BackgroundGlobally, prematurity is the leading cause of death in children under the age of 5. Many efforts have focused on clinical approaches to improve the survival of premature babies. There is a need, however, to explore psychosocial, sociocultural, economic, and other factors as potential mechanisms to reduce the burden of prematurity. Women's empowerment may be a catalyst for moving the needle in this direction. The goal of this paper is to examine links between women's empowerment and prematurity in developing settings. We propose a conceptual model that shows pathways by which women's empowerment can affect prematurity and review and summarize the literature supporting the relationships we posit. We also suggest future directions for research on women's empowerment and prematurity.MethodsThe key words we used for empowerment in the search were "empowerment," "women's status," "autonomy," and "decision-making," and for prematurity we used "preterm," "premature," and "prematurity." We did not use date, language, and regional restrictions. The search was done in PubMed, Population Information Online (POPLINE), and Web of Science. We selected intervening factors-factors that could potentially mediate the relationship between empowerment and prematurity-based on reviews of the risk factors and interventions to address prematurity and the determinants of those factors.ResultsThere is limited evidence supporting a direct link between women's empowerment and prematurity. However, there is evidence linking several dimensions of empowerment to factors known to be associated with prematurity and outcomes for premature babies. Our review of the literature shows that women's empowerment may reduce prematurity by (1) preventing early marriage and promoting family planning, which will delay age at first pregnancy and increase interpregnancy intervals; (2) improving women's nutritional status; (3) reducing domestic violence and other stressors to improve psychological health; and (4) improving access to and receipt of recommended health services during pregnancy and delivery to help prevent prematurity and improve survival of premature babies.ConclusionsWomen's empowerment is an important distal factor that affects prematurity through several intervening factors. Improving women's empowerment will help prevent prematurity and improve survival of preterm babies. Research to empirically show the links between women's empowerment and prematurity is however needed
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